Cinquin Lab

Control of Caenorhabditis elegans germ-line stem-cell cycling speed meets requirements of design to minimize mutation accumulation.

Chiang M., Cinquin A., Paz A., Meeds E., Price C.A., Welling M., Cinquin O. BMC Biology 13:51 (2015) Abstract BACKGROUND: Stem cells are thought to play a critical role in minimizing the accumulation of mutations, but it is not clear…

Live imaging reveals active infiltration of mitotic zone by its stem cell niche.

Wong B.G.‡, Paz A.‡, Corrado M.A., Ramos B.R., Cinquin A., Cinquin O.*, Hui E.E.* Integrative Biology 5(7), pp976-982 (2013)   Abstract Stem cells niches are increasingly recognized as dynamic environments that play a key role in transducing signals that allow…

Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue.

Stringari C., Cinquin A., Cinquin O., Digman M.A., Donovan P.J., Gratton E. PNAS 108(33), pp13582-7 (2011) Abstract We describe a label-free imaging method to monitor stem-cell metabolism that discriminates different states of stem cells as they differentiate in living tissues.…

Progression from a stem cell–like state to early differentiation in the C. elegans germ line

Cinquin O‡*, Crittenden S.L.‡, Morgan D.E., Kimble J*. PNAS 107(5), pp2048-53 (2010) Abstract Controls of stem cell maintenance and early differentiation are known in several systems. However, the progression from stem cell self-renewal to overt signs of early differentiation is…

A.3 Simulations

Simulations were performed with the Numerical Algorithms Group’s Fortran library Mark 20, using the D03PDF function, called from a custom Fortran 77 program (available on request), compiled with the NAGWare compiler, and run on G5 iMacs and PowerMacs. The D03PDF…

A.2 Evaluation of Gradients

Small dependence of the gradient on the rate of morphogen production has been used as a criterion of biological relevance (Eldar et al., 2003). There are however documented cases of morphogen readouts not being buffered against changes in morphogen production…

A.1 Parameter Values

Table 1: Ranges from which parameters were selected at random for the simulations described in section 3. Parameters Range Distribution Log-uniform 5-30 Uniform Log-uniform Log-uniform Log-uniform log-uniform in Log-uniform , log-uniform in Log-uniform The concentrations of morphogens and receptors, as…

Appendix

Subsections Parameter values Evaluation of gradients Simulations Equations for Wg-like diffusion Equations for Shh-like oligomerization Ranges for the measures plotted in figures 5 and 6

A new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an Otx2 enhancer between mouse and chick extraembryonic endoderm. Full Text

Amanda Albazerchi1,2 , Olivier Cinquin1,3 and Claudio D Stern1 1Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT. UK 2Laboratory of Genetics, 425-G Henry Mall, University of Wisconsin, Madison, WI 53706, USA 3Deparment of Biochemistry,…

Understanding the somitogenesis clock: what’s missing?

Cinquin O. Mech. Dev. 124(7-8), pp501-517 (2007) Abstract The segmentation of vertebrate embryos depends on a complex genetic network that generates highly dynamic gene expression. Many of the elements of the network have been identified, but their interaction and their…

A new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an Otx2 enhancer between mouse and chick extraembryonic endoderm.

Albazerchi A., Cinquin O., Stern C.D. BMC Dev. Biol. 7:25 (2007) Abstract BACKGROUND: The mouse anterior visceral endoderm (AVE) and the chick hypoblast are thought to have homologous roles in the early stages of neural induction and primitive streak formation.…

High-dimensional switches and the modeling of cellular differentiation

Cinquin O., Demongeot J., J. Theor. Biol. 233(3), pp391-411 (2005) Abstract Many genes have been identified as driving cellular differentiation, but because of their complex interactions, the understanding of their collective behaviour requires mathematical modelling. Intriguingly, it has been observed…

Is the somitogenesis clock really cell-autonomous? A coupled-oscillator model of segmentation

Cinquin O., J. Theor. Biol. 222(4), pp459-468 (2003) Abstract A striking pattern of oscillatory gene expression, related to the segmentation process (somitogenesis), has been identified in chick, mouse, and zebrafish embryos. Somitogenesis displays great autonomy, and it is generally assumed…

Identification, cloning and functional expression of the gene encoding OMP decarboxylase from Plasmodium falciparum

Menz R.I., Cinquin O., Christopherson R.I. Ann. Trop. Med. Parasitol. 96(5), pp469-476 (2002) Abstract The coding region of a putative orotidine 5′-monophosphate decarboxylase gene from Plasmodium falciparum was identified in genomic data from the Malarial Genome Sequencing Project. The gene…