Cinquin Lab

Identification, cloning and functional expression of the gene encoding OMP decarboxylase from Plasmodium falciparum

Menz R.I., Cinquin O., Christopherson R.I. Ann. Trop. Med. Parasitol. 96(5), pp469-476 (2002)

Abstract

The coding region of a putative orotidine 5′-monophosphate decarboxylase gene from Plasmodium falciparum was identified in genomic data from the Malarial Genome Sequencing Project. The gene encodes a protein of 323 amino acids with a predicted molecular weight of 37.8 kDa. The gene was cloned into a bacterial expression vector and over-expressed in Escherichia coli. The recombinant protein was purified and shown to have orotidine 5′-monophosphate decarboxylase activity, confirming the identity of the gene.