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Next: Acknowledgments Up: Is the somitogenesis clock Previous: Effect of L-fng misexpression

Conclusion

The Lunatic fringe secretion model can account for the oscillatory gene expression pattern exhibited by the PSM of chick and mouse embryos. It is dependent on local coupling between cells, which allows it for example to not require new cells to ingress into the PSM with an oscillatory initial phase, and to be more resistant to random perturbations. However, oscillations also have an autonomous character, in that the introduction of a coupling boundary at a specific position of the PSM does not significantly affect the oscillatory pattern. Such a behaviour is in agreement with experiments which were previously interpreted as ruling out the existence of coupling between cellular oscillators.

This shows that coupling between the PSM oscillators is compatible with all current experimental data. What's more, such coupling could explain phenomena which have hitherto remained obscure. The model could benefit from an extension to 2 or 3 dimensions, to earlier embryonic times (and explain the way the phase gradient is set up by the spread of the very first wave), and to a strength of coupling set by FGF8 levels (which could then allow to explain the anisotropic effect of FGF8-beads grafts described by Dubrulle et al., 2001). This will be addressed in later studies.

Finally, the model, even though it is based on L-fng activation of Notch signalling (in agreement with biological studies of L-fng), is also compatible with data interpreted as supporting L-fng-mediated repression of Notch signalling.

Note added in proof

After this article went to press, J. Lewis proposed a model for the somitogenesis clock, based on negative feedback and transcriptional delays, inspired by the zebrafish but applicable to chick and mouse (Lewis, 2003). Experiments will be required to distinguish between this model and the Lunatic fringe secretion model, but they could differ in their synchronisation properties, and in their abilities to account for the differences in oscillatory patterns in anterior and posterior PSM.
next up previous
Next: Acknowledgments Up: Is the somitogenesis clock Previous: Effect of L-fng misexpression
Olivier Cinquin 2003-04-14